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  Indian J Med Microbiol
 

Figure 2: General mechanism of clustered regularly interspaced short palindromic repeat-Cas9 system and its three stages: (1) Viral DNA consists of a unique PAM sequence which is integrated into the CRISPR loci (2) Transcription takes place and crRNA is formed (3) crRNA binds with the cas protein and causes break down of viral genome. When the viral DNA enters the bacterial cell, the protospacer and unique PAM sequence help guide RNA and Cas9 to locate the sequence, followed by transcription of crRNA.

Figure 2: General mechanism of clustered regularly interspaced short palindromic repeat-Cas9 system and its three stages: (1) Viral DNA consists of a unique PAM sequence which is integrated into the CRISPR loci (2) Transcription takes place and crRNA is formed (3) crRNA binds with the cas protein and causes break down of viral genome. When the viral DNA enters the bacterial cell, the protospacer and unique PAM sequence help guide RNA and Cas9 to locate the sequence, followed by transcription of crRNA.